As the bottle is factory-sealed for a long shelf life, it is recommenced to use a medical syringe to get the product our of the bottle . By inserting the syringe through the lid, the product will not have any contact with oxygen and will not loose the qualities for long time. One ampoule of 20 ml is enough for almost 150 treatments. 

1) Before the treatment - place the ampoule in a glass with hot water for a minute or two to warm up the botox. 

2) Insert a medical insulin syringe 1ml and get 0.1ml of the substance out of the bottle.

3) Pour the 0.1 ml of the essence into a glass dish

4) Add 1ml of hot water to the same glass dish Mixing ratio is 1:10 (1 Botox, 10 water. 

5) Mix the essence and hot water thoroughly until smooth with a micro brush or mixing tool. 

6) Apply the mixture on eyebrows and cover with cling wrap. Processing times 15 minutes. 

7) Lift the wrap every 5 minutes and brush the hairs with a clean disposable brush letting them fill in with oxygen. Cover with the wrap again.

8) Remove the wrap and clean the eyebrows with a damp cotton pad. The procedure should be performed BEFORE eyebrow tinting. 

9) Apply the remaining Botox (a size of a rice grain per brow) after you finish the treatment as a leave - in solution. 

It is also a perfect addition to your signature Brow lamination treatment:

Perform Step 1 and Step 2 first, apply Brow botox and cover the eyebrows for 15 minutes after Step 2, remove the essence with a damp cotton pad and proceed with eyebrow tinting.

Apply Step 3 as the last step after you remove the tint. 

The second procedure of O2 Gold must be repeated after 20-25 days.